R&D Study of Micro-Scale Heat and Fluid Flow System for Cell Processing
N. Kasagi, Y. Suzuki, T. Wu, S. Hashimoto, N. Chen, H. Nomoto
Overview
With the rapid progress in micro-fabrication techniques, development of
miniature devices for bio-chemical analyses is becoming a major topic in
research fields such as chemical engineering, and bio engineering. These
devices are integrated into small on-chip systems, which are called
micro-TAS (micro-total-analysis-system) or Lab-on-a-chip. Realization of
Lab-on-a-chips lead to reduction of sample volume and processing time,
increase in efficiency, and manpower saving.
In most kinds of bio-chemical analyses, handling of sample solutions and
temperature control are very important issues. These aspects are even more
important in micro-scale devices, where fluid motion is known to show
different behavior compared to flow in large-scale devices. Therefore,
proposing micro-scale biofluidic devices designed from the viewpoint of
thermo-fluid dynamics will provide new insights to the micro-TAS field.
The target of the present study is to develop a cell selection system
that separates desired types of cells from biological fluids like
peripheral blood or bone marrow, in a micro conduit. We develop chaotic
mixers for selection of cells by use of antigen/antibody reactions,
magnetic separators, and micro pumps. Numerical simulations and 3-D
microscopic digital image measurements of velocity, concentration and
temperature of flows in those devices are also performed.
Collaborator: T. Ushida, K. Furukawa
Sponsor: Grant-in-Aid for Science Research (S) (MEXT) [PI: N. Kasagi]
Micro immunomagnetic cell sorter
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Micro column for adhesion-based cell separation
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Recent results
(Review)
- Miwa, J., Tan, W. H., Suzuki, Y., Kasagi, N., Shikazono, N., Furukawa,
K. and Ushida, T.,
"Development of micro immunoreaction-based cell sorter for
regenerative medicine,"
Proc. 1st Intl. Conf. BNI Fusion, Marina del Rey, CA, USA, Jul. 20-22,
2005.
(PDF 2.0 Mbytes)
(Micro mixer)
- Inokuchi, H., Nagae, K., Suzuki, Y., Kasagi, N., and Shikazono, N.,
"Evaluation of Lamination Micro Mixer for Micro Immunomagnetic Cell Sorter,"
Proc. 2006 Int. Conf. Microtechnologies in Medicine and Biology, Okinawa, Japan, May 9-12, 2006, pp. 131-134.
- Tan, W.-H., Suzuki, Y., Kasagi, N., Shikazono, N., Furukawa, K., and Ushida, T.,
"Lamination Micro Mixer for Micro-immunomagnetic Cell Sorting,"
Int. J. JSME, Ser. C, Vol. 48, No. 4, (2005), pp. 425-435.
- Suzuki, H., Ho., C.-M., and Kasagi, N.,
"A Chaotic Mixer for Magnetic Beads-Based Micro Cell Sorter,"
J. Microelectromech. Sys., Vol. 13, No. 5, pp. 779-790 (2004).
(doi:10.1109/JMEMS.2004.835775)
- Fukagata, K., Kasagi, N., and Chaktranond, C.,
"Lagrangian chaos in a micro serpentine mixer for immunomagnetic cell sorting,"
Proc. Transport Phenomena in Micro and Nanodevices, October 2004, Kona Coast, Hawaii (CD-ROM), Paper No. ii-5, 6 pp.
(PDF 1.8 Mbytes).
(Separator)
- Inokuchi, H., Suzuki, Y., Kasagi, N., Shikazono, N., Furukawa, K., and Ushida, T.,
"Micro Magnetic Separator for Stem Cell Sorting System,"
Proc. 22nd Sensor Symp., Tokyo, Oct. 20-21, 2005, pp. 125-128.
(PDF 737 kbytes)
(Adhesion-based cell sorting)
- Miwa, J., Suzuki, Y. and Kasagi, N.,
"Adhesion-Based Cell Sorter with Antibody-Immobilized Functionalized-Parylene Surface,"
Proc. Int. Conf. IEEE MEMS 2007, Kobe (2007), pp. 27-30.
(PDF 1.0 Mbytes)
(Micro PIV)
- Angele, K. P., Suzuki, Y., Miwa, J., and Kasagi, N.,
"Development of
High-Speed Micro Scanning PIV Using a Rotating Disk,"
Meas. Sci. Tech, Vol. 17, No. 7, (2006), pp. 1639-1646.
(PDF 920 kbytes,
doi:10.1088/0957-0233/17/7/001
)
Last update: 2007-1-30